Proteins Specified by Herpes Simplex Virus

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Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.

We are reporting a procedure for the purification of herpes simplex enveloped nucleocapsids (virions), an evaluation of the purification procedure and the results of analyses of the virion proteins by high-resolution acrylamide gel electrophoresis. The data may be summarized as follows. (i) The procedure for the purification of virions consists of careful extraction of cytoplasm to prevent nucl...

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Structural proteins of herpes simplex virus.

Herpes simplex virus, grown in BHKzI cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodec...

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Detection of Herpes Simplex Virus Infection in Cerebrospinal Fluid

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1970

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.5.2.123-131.1970